Which assay utilizes a non-specific protein binding dye for identification?

The Bradford assay is recognized for its use of a non-specific protein-binding dye, namely Coomassie Brilliant Blue. This dye interacts with proteins, leading to a color change that can be quantitatively measured to determine protein concentration. The interaction primarily involves the dye binding to the amino acids of the proteins, and since this binding is not restricted to specific proteins, the assay is considered non-specific. This characteristic allows for a rapid and straightforward technique for protein quantification in various samples.

In contrast, the Lowry assay and BCA assay involve more specific interactions and chemical reactions related to particular amino acid residues. The Western blot technique, while an essential method for protein identification, relies on specific antibodies to target particular proteins, rather than a dye for general quantification. Thus, the Bradford assay stands out for its non-specific binding capabilities among the listed options.