What does the 2nd stop of a micropipette accomplish?

The function of the second stop on a micropipette is to release the liquid contained in the tip. When aspirating a liquid, the first stop is used to draw the liquid into the pipette, and during the dispensing process, the second stop ensures that all the liquid is accurately released from the tip. This mechanism is crucial for precision in laboratory settings, as it allows the user to expel the exact volume of liquid required without leaving residual liquid in the tip. This accuracy is vital for experiments where precise measurements of reagents are necessary, emphasizing why understanding the function of the second stop is important in pipetting techniques.