What does the 1st stop on a micropipette refer to?

The first stop on a micropipette refers to the position of the plunger that allows the user to fill the tip with liquid. When you press the plunger down to this first stop, the mechanism creates a vacuum in the pipette tip that draws liquid into the tip. This action is crucial for accurate liquid measurement, as it ensures that the exact volume intended can be aspirated into the pipette. Understanding this function is vital for proper pipetting technique to avoid errors in volume measurement during experiments.